We collected 1001 blood samples from Egyptian and Algerian camels: 600 from Egypt (370 samples from Marsa Matrouh, 100 samples from Aswan, 30 samples from Giza, 100 samples from Sharqia) and 401 samples from Algeria (Table S1A). Blood samples were collected in vacutainer tubes containing ethylenediaminetetraacetic acid (EDTA). Assessment of oxidant/antioxidant markersThe oxidant/antioxidant assessment was evaluated only in the Egyptian blood samples. The positive PCR samples for Trypanosoma (− ve Babesia and −ve Theilaria) were selected only to estimate oxidant/antioxidant parameters. Individual reactions were performed in 25 μl volumes consisting of 1 μl DNA, 10 μl PCR master mix (2x), 9 μl PCR water, 0.5 μl forward primer (10 μM), 0.5 reverse primer (10 μM), and 4 μl blocking primer (10 μM) (Thermo Fisher Scientific Inc.).

Source: The North Africa Journal May 03, 2023 09:40 UTC