Prosser, WA In the pursuit of enhancing almond genetic diversity for the ever-expanding global demand, researchers have delved into a private almond germplasm collection, shedding light on the potential for new cultivars adapted to diverse growing conditions. The study compared the genetic diversity of the private IPPFBE germplasm collection, which focuses on Central Asian origins, with the existing NPGS collection maintained near Davis, California. Through DNA fingerprinting using 10 simple sequence repeat markers, this research revealed insights into the diversity of these almond accessions. Two Prunus bucharica accessions from the IPPFBE collection stood out, displaying distinctive genetic traits compared to the rest of the collection. Analysis of the IPPFBE collection indicates that although it and the NPGS collection are broadly similar, the new material contributes positively to the genetic diversity of almond germplasm.